Research Articles
Inter-Platform Reproducibility in DNA Methylation Detection: A Comprehensive Guide for Robust Epigenetic Research
This article provides a systematic evaluation of the reproducibility and reliability of DNA methylation detection across major technological platforms, including bisulfite sequencing, microarrays, and emerging long-read and enzymatic methods.
Bisulfite Genomic Sequencing: Validating the Gold Standard for DNA Methylation Analysis in Biomedical Research
This article provides a comprehensive validation of bisulfite genomic sequencing (BS-seq) as the gold standard for DNA methylation analysis.
Cross-Platform Validation in DNA Methylation Analysis: Bridging Microarrays and Sequencing for Robust Clinical Diagnostics
This article provides a comprehensive analysis of the cross-platform validation strategies essential for translating DNA methylation biomarkers from research to clinical practice.
RRBS vs. EpiTyper: A Strategic Guide for Comparing Bisulfite Sequencing Platforms in Biomedical Research
This article provides a comprehensive comparison of two prominent DNA methylation analysis platforms: Reduced Representation Bisulfite Sequencing (RRBS) and the Sequenom EpiTyper.
A Comprehensive Guide to Validating RRBS DNA Methylation Data: From Foundational Principles to Clinical Application
This article provides a systematic framework for validating Reduced Representation Bisulfite Sequencing (RRBS) data, tailored for researchers and drug development professionals.
Discriminating True SNPs from Bisulfite Conversion Artifacts: A Comprehensive Guide for Genomic Researchers
Accurately discriminating true single nucleotide polymorphisms (SNPs) from artifacts introduced by bisulfite conversion is a critical challenge in epigenomic studies.
Batch Effect Correction in Bisulfite Sequencing: A Comprehensive Guide for Robust Epigenetic Analysis
This article provides a comprehensive guide for researchers and drug development professionals on addressing batch effects in bisulfite sequencing datasets.
Normalization and Binning Procedures for Variable Region Sizes: A Comprehensive Guide for Biomedical Data Analysis
This article provides a comprehensive guide for researchers and drug development professionals on the critical role of normalization and binning procedures in the analysis of high-dimensional biomedical data with variable...
Navigating the Challenges: A Comprehensive Guide to Methylation Calling Accuracy in Low Coverage Regions
Accurate DNA methylation calling in low-coverage sequencing data remains a significant challenge in epigenomic studies, impacting cost-efficiency and data reliability.
A Comprehensive Guide to BS-seq Data Quality Control: Best Practices for Pre-alignment and Post-alignment Analysis
This article provides a complete guide to quality control (QC) for Bisulfite Sequencing (BS-seq) data, a gold-standard method for DNA methylation analysis.