Research Articles
From Discovery to Validation: A Comprehensive Guide to Integrating RNA-Seq and qPCR in Biomedical Research
This article provides a complete roadmap for researchers and drug development professionals navigating the transition from genome-scale RNA-Seq discovery to targeted qPCR validation.
Low Abundance Gene Quantification: RNA-Seq vs qPCR - A Researcher's Guide to Accuracy and Application
Accurately quantifying low-abundance transcripts is critical for advancing research in drug development, biomarker discovery, and understanding complex disease mechanisms.
Beyond Housekeeping: The Critical Role of Reference Genes in Accurate qPCR Normalization
Quantitative real-time PCR (RT-qPCR) is a cornerstone of gene expression analysis in biomedical and biological research, but its accuracy is entirely dependent on proper normalization.
From Sequencing to Confirmation: A Comprehensive Guide to qPCR Validation of RNA-Seq Data
This article provides a definitive guide for researchers and drug development professionals on validating RNA-Seq findings with qPCR.
MIQE Guidelines for qPCR Validation: Ensuring Reproducibility in Research and Drug Development
This article provides a comprehensive guide to the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines, a critical framework for ensuring the reproducibility and reliability of qPCR...
qPCR Validation for Transcriptomics: When It's Required and When It's Not
This article provides a definitive guide for researchers and drug development professionals on the role of qPCR validation in transcriptomics studies.
Bridging the Gap: A Comprehensive Guide to RNA-Seq and qPCR Correlation in Biomedical Research
This article provides a comprehensive framework for researchers and drug development professionals on correlating RNA-Seq and qPCR data, the established gold standard for gene expression validation.
RNA-Seq Validation with qPCR: A Strategic Guide for Biomedical Researchers
This article provides a comprehensive framework for researchers and drug development professionals on the critical role of qPCR in validating RNA-Seq data.
Adjacent CpG Methylation Concordance: From Foundational Principles to Clinical Applications in Biomarker Discovery
This article provides a comprehensive exploration of methylation level concordance at adjacent CpG sites, a critical aspect of epigenetic regulation with profound implications for cellular identity, disease mechanisms, and biomarker...
Sensitivity and Specificity in DNA Methylation Detection: A 2025 Guide for Biomarker Validation and Clinical Translation
This article provides a comprehensive guide for researchers and drug development professionals on evaluating the sensitivity and specificity of DNA methylation detection methods.